Brand: Sigma-Aldrich/MilliporeSigma · SKU: P5412 · MPN: P5412 · Category: Components
o-Phenylenediamine (OPD) is a chromogenic substrate suitable for use in ELISA procedures which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 M HCl or 3 M H2SO4, and read at 492 nm. The oxidation product is 2,3-diaminophenazine. This product is supplied as 50 or 100 tablets per box, individually foil wrapped for ease of use, storage, and safety. Each tablet weighs ~45 mg (range 40–50 mg) and contains 20 mg of substrate. One tablet, dissolved in 10 mL of water, gives a solution with a pH of 9.0 (range 8.5–9.5). The background absorbance of this solution cannot be more than 0.04. This product is for R&D use only, not for drug, household, or other uses. Store tablets at 2–8 °C. Protect from heat, light and moisture. Allow tablets to reach room temperature before use. Solutions should be freshly prepared. For preparation, dissolve
| Form | Tablet |
|---|---|
| Synonyms | 1,2-Benzenediamine, 1,2-Diaminobenzene, OPD |
| EC Number | 202-430-6 |
| CAS Number | 95-54-5 |
| Boiling Point | 256-258 °C |
| Melting Point | 100-102 °C |
| Molecular Weight | 108.14 |
| Molecular Formula | C6H8N2 |
| Storage Temperature | 2-8°C |
| Warranty | Not Available |
| Weight | ~45 mg per tablet |
| Dimensions | Not Available |
o-Phenylenediamine (OPD) is a chromogenic substrate for ELISA procedures using horseradish peroxidase conjugates, producing an orange-brown soluble end product readable spectrophotometrically at 450 nm or 492 nm after stopping the reaction. It is supplied in foil-wrapped tablets containing 20 mg of substrate each.
Yes, this o-Phenylenediamine tablet, 20 mg substrate per tablet (P5412-50TAB) (P5412) is 100% genuine Sigma-Aldrich/MilliporeSigma hardware. All our products are verified by certified technicians and come with clean serial numbers ready for service contract registration.
This o-Phenylenediamine tablet, 20 mg substrate per tablet (P5412-50TAB) includes our standard 3-Year Warranty covering all manufacturing defects. We also offer advanced replacement options for minimal downtime.
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Yes, we have a 30-day return policy on all products. Restocking fees may apply for opened items. Please see our return policy for complete details.
Please check the specifications tab for detailed compatibility information. Our technical team is available to assist with compatibility questions for the P5412.